The field of genome editing is experiencing a renaissance driven primarily by the repurposing of an immune response system utilized by bacteria and archaea. This system is characterized by the presence of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) stored in host genomes as memories of phage-bacteria interactions. The use of the CRISPR system in higher organisms (including mammalian cells and animal models) has spurred myriads of applications critically relevant to agriculture, biomanufacturing, and human health. We discuss results at the interface of synthetic biology and genome editing with particular emphasis on engineering biological networks with applications in gene therapy.
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